Free radicals clog peripheral blood vessels , leading to the brain causing cerebral thrombosis , cerebral hemorrhage , etc 腦與心病癥活性氧使周圍靜脈阻塞造成功能失常腦血栓形成腦出血等。
Objective : to establish a rat model of orthotopic gastric isotransplantation by using microsurgical techniques . methods : 70 sd rats were used in our experiment and 35 gastric trasplantations were carried out . in the donor ' s operation : after the spleen was resected and the proper liver artery was ligated , the stomach was perfused through the abdominal aorta . then the stomach was resected with its peripheral blood vessels including celiac trunk and the portal vein , etc . in the recipient operation : after the stomach and the spleen were reseeted , the implantation was performed by the following sequence : the end - to - side anastomosis between the portal veins . the end - to - end anastomosis between the celiac trunk and the left gastric artery . open the blood flow to observe the effect of the blood supply of the stomach . the end - to - end anastomosis between the duodenum . the end - to - end anastomosis between the cardiac and the esophagus . results : 35transplantations were carried out in which the operation success rate in the last 20 cases was 80 ( 16 / 20 ) . the average operation time was 2 . 35 h . the longest survival time was over three months . conclusions : the model of orthotopic gastric transplantation in rat was successfully established . it could be used to study the transplanted stomach in the abdominal multiviseeral transplantation and the reconstruction after the total gastrectomy 目的應(yīng)用顯微外科技術(shù),建立大鼠原位異體胃移植模型.方法70只sd大鼠,行35例次的胃移值手術(shù).供體手術(shù),先切除脾臟,經(jīng)腹主動脈行原位胃冷灌洗.將胃及其所屬血管,包括腹腔干和門靜脈干等一并切取.受體手術(shù),先切除胃和脾臟,分別行供、受體間門靜脈的端側(cè)吻合,供體腹腔干與受體胃左動脈的端端吻合,然后開放血流.再行供體和受體十二指腸間端端吻合,賁門與食管端端吻合.結(jié)果在施行的35例手術(shù),后20例中有16例成功,成功率為80 .最長存活者達(dá)3個月.結(jié)論成功地建立了大鼠原位異體胃移植類型.該模型可用于腹部多臟器移植中移植胃的相關(guān)研究及全胃切除術(shù)后代胃的研究